Raspaje y alisado radicular en pacientes fumadores con periodontitis crónica moderada-severa: efectos clínicos y microbiológicos / Scaling and Root Planing in smoker patients with Moderate to Severe Chronic Periodontitis: Clinical and microbiological effects

Objetivo: Se realizó un estudio piloto para determinar la eficacia, a nivel clínico y microbiológico, del raspaje y alisado radicular en una sesión vs. varias sesiones, y la comodidad ante el tratamiento, en pacientes fumadores con periodontitis crónica moderada a severa. Materiales y Métodos: Ingresaron nueve pacientes fumadores, de ambos sexos, entre 40 y 70 años, sistémicamente sanos, con bolsas periodontales ≥4mm, pérdida de inserción, sangrado al sondaje, diagnosticados con periodontitis crónica moderada a severa según los criterios de la American Academy of Periodontology (A.A.P.). Fueron asignados aleatoriamente a dos grupos, para raspaje y alisado radicular, grupo A en una sesión, Grupo B a intervalos de una semana por cada cuadrante. Se midió la profundidad de bolsa, el nivel de inserción clínico, sangrado al sondaje; se tomaron muestras de placa bacteriana subgingival para cultivo microbiológico pre y seis meses después del tratamiento; se indagó acerca de la comodidad durante el procedimiento con una encuesta. Resultados: De las variables clínicas analizadas, en grupo A se observó disminución de profundidad de bolsas en 0.88mm, nivel de inserción clínico en 1.12mm, sangrado al sondaje en 7.52% respecto a los promedios iniciales; en grupo B, no se observaron diferencias respecto a las mediciones iniciales en contraste con el sangrado al sondaje que disminuyó en 0,50%. En ambos grupos se encontró reducción en la prevalencia de: Aggregatibacter actinomycetemcomitans (33%), Porphyromonas gingivalis (22%), Prevotella intermedia (22%), Fusobacterium species (55%), Eikenella corrodens (44%), Bacilos entéricos Gram negativos (11%). El grupo B refirió mayor comodidad que el grupo A durante el tratamiento. Conclusiones: Aunque no hubo diferencias estadísticamente significativas, hubo una tendencia que mostró que el raspaje y alisado radicular en una sesión obtuvo mejor eficacia clínica y microbiológica que el raspaje y alisado radicular por sesiones, aunque mostró menor comodidad durante el procedimiento.--(AU)

Aim: A pilot clinical trial was performed to determine (i) microbiological and clinical efficacy (ii) confort of One Stage scaling and root planing vs. multiple SRP sessions, in smoker patients with chronic periodontitis. Materials and Methods: Nine smoker patients, men and women, between 40 to 70 years old, systemically health, with mean deep pocket ≥4mm, attachment loss, bleeding on probing, diagnosed with chronic periodontitis moderate to severe based on American Academy of Periodontology (A.A.P.), were randomized into two groups for scaling and root planing, group A for full-mouth, and group B per one week. Deep pocket, clinical attachment lost, bleeding on probing was measured. Subgingival samples were taken from deepest pockets before and six months after treatment. Patients were asked with a survey about comfort of the treatment in both groups. Results: Group A showed mean deep pocket decreased in 0.88mm, clinical attachment lost in 1.12mm, bleeding 7.52% initial average respect. Group B, did not show changes respect initial measures, in contrast with bleeding, it has decreased in 0.5%. Both groups had showed similar decrease in prevalence of: Aggregatibacter actinomycetemcomitans (33%), Porphyromonas gingivalis (22%), Prevotella intermedia (22%), Fusobacterium species (55%), Eikenella corrodens (44%), Gram negative enteric rods (11%). Group B has referred better comfort than group A, during the treatment. Conclusions: Although there was no statistically significant differences, there was a trend showing that the Full-mouth scaling and root planning got better clinical and microbiologicalefficacy than scaling and root planing in multiple sessions, but had shown lower comfort around the treatment...(AU)

Cultivo y Caracterización In Vitro de una Línea Celular de Fibroblastos Gingivales Humanos / In vitro culture and characterization of a cell line of human gingival fibroblasts

The main goal is to establish a cellular line of fibroblast which come from human gingival tissue, identifying its morphologic featores. Samples were taken from gum of healthy patients without medical antecedents. Three females and three male were selected. Ages were between 16 and 30 years old. A sample of 0,5 cm² of gum was obtained to take it to a cellular growth environment. Cultures were incobated at 37C, CO² atmosphere at 5% for ten (10) days. Samples were observed daily with inverted light Microscope Olimpus II. At 6th day growth of fusiform cells was observed; their morphology was in coincidence with that of the fibroblasts, At 18th day a monolayer of 100% purity was observed. The cellular line was preserved and stored by freezing in liquid nitrogen.